Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ABIN1607875 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-H2A Histone Family, Member X (H2AFX) (C-Term) antibody
- Antibody type
- Polyclonal
- Description
- H2AX pS139 is directed against the phosphorylated form of human H2AX protein at the pS139 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity with non-phosphorylated human H2AX is minimal by ELISA and western blot.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 100 μg
- Storage
- Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Handling
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- WB