600-401-H36

antibody from Rockland Immunochemicals, Inc.

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-H36

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-H36, RRID:AB_2614306

Product name

Anti-H2AX pS139 (RABBIT) Antibody - 600-401-H36

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Molecular and Biochemical Methods Useful for the Epigenetic Characterization of Chromatin-Associated Proteins in Bivalve Molluscs.

Rivera-Casas C, Gonzalez-Romero R, Garduño RA, Cheema MS, Ausio J, Eirin-Lopez JM
Frontiers in physiology 2017;8:490

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 µg per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none.

Validation comment

Western Blot