613410

antibody from BioLegend

Targeting: H2AX H2AFX

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

613410

Provider

BioLegend

Proper citation

BioLegend Cat#613410, RRID:AB_2616774

Product name

Alexa Fluor® 594 anti-H2A.X-Phosphorylated (Ser139)

Antibody type

Monoclonal

Antigen

Modified peptide

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

2F3

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of Alexa Fluor® 594 anti-H2A.X Phospho (Ser139) (clone 2F3) (red) in 5% FBS overnight followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.

Conjugate

Yellow dye