613416

antibody from BioLegend

Targeting: H2AX H2AFX

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

613416

Provider

BioLegend

Proper citation

BioLegend Cat#613416, RRID:AB_2629534

Product name

APC anti-H2A.X-Phosphorylated (Ser139)

Antibody type

Monoclonal

Antigen

Modified peptide

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antibody clone number

2F3

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Nocodozole treated HeLa cells (24 hours) were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with anti-H2A.X Phospho (Ser139) (clone 2F3) APC (filled histogram) or mouse IgG1, κ APC (open histogram).

Conjugate

Red dye