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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- MAB12265 - Provider product page
- Provider
- Abnova Corporation
- Product name
- TIE1 monoclonal antibody, clone 8D12B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human TIE1.
- Isotype
- IgG
- Antibody clone number
- 8D12B10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human TIE1 (AA: 385-607) recombinant protein (Expected MW is 50.6 kDa) with TIE1 monoclonal antibody, clone 8D12B10 (Cat# MAB12265) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cells] with TIE1 monoclonal antibody, clone 8D12B10 (Cat# MAB12265) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HepG2 cell lysate with TIE1 monoclonal antibody, clone 8D12B10 (Cat# MAB12265) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of TIE1 monoclonal antibody, clone 8D12B10 (Cat# MAB12265) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human liver cancer tissues with TIE1 monoclonal antibody, clone 8D12B10 (Cat# MAB12265) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
