Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-41358 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal beta COP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta COP Antibody [NBP2-41358] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB1 antibody NBP2-41358 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: beta COP Antibody [NBP2-41358] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB1 antibody NBP2-41358 used for IP at 6 ug per reaction. COPB1 was also immunoprecipitated by rabbit anti-COPB1 antibody NBP2-41358. For blotting immunoprecipitated COPB1, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.