PA5-27968

antibody from Invitrogen Antibodies

Targeting: OAT HOGA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27968

Provider

Invitrogen Antibodies

Product name

OAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: K562, THP-1, Mouse brain, OAT-transfected 293T.

Concentration

0.28 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OAT using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an OAT polyclonal antibody (Product # PA5-27968) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of OAT was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a OAT Polyclonal Antibody (Product # PA5-27968) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using OAT Polyclonal Antibody (Product # PA5-27968). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. OAT Polyclonal Antibody (Product # PA5-27968) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of OAT was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a OAT Polyclonal Antibody (Product # PA5-27968) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of OAT was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: OAT Polyclonal Antibody (Product # PA5-27968) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using OAT (Product # PA5-27968) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.