PA5-79562

antibody from Invitrogen Antibodies

Targeting: KLK1 Klk6

Antibody data

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Validation data

Product number: PA5-79562 - Provider product page
Provider: Invitrogen Antibodies
Product name: Kallikrein 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 500 µg/mL
Storage: -20°C

KLK1 protein structure - PA5-79562 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Kallikrein 1 in Lane 1: recombinant human KLK1 protein, Lane 2: recombinant human KLK1 protein 5 ng Lane 3: recombinant human KLK1 protein using 10, 5 and 2.5 ng, respectively per well. Sample was incubated with Kallikrein 1 (Product # PA5-79562).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Kallikrein 1 in Lane 1: recombinant human KLK1 protein, Lane 2: recombinant human KLK1 protein 5 ng Lane 3: recombinant human KLK1 protein using 10, 5 and 2.5 ng, respectively per well. Sample was incubated with Kallikrein 1 (Product # PA5-79562).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLK1 in, Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates, . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with Kallikrein 1 Polyclonal Antibody (Product # PA5-79562) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for KLK1 at approximately 29KD. The expected band size for KLK1 is at 29KD.