TA500527

antibody from OriGene

Targeting: FRK GTK, PTK5, RAK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500527

Provider

OriGene

Proper citation

OriGene Cat#TA500527, RRID:AB_2106069

Product name

FRK mouse monoclonal antibody, clone OTI7H3 (formerly 7H3)

Antibody type

Monoclonal

Description

FRK mouse monoclonal antibody, clone OTI7H3 (formerly 7H3)

Host

Mouse

Conjugate

Unconjugated

Epitope

FRK

Isotype

IgG

Antibody clone number

OTI7H3

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500527)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC204460 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FRK antibody(TA500527), and then analyzed by flow cytometry.

Validation comment

FC