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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX83989 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83989, RRID:AB_10731671
- Product name
- NT5DC1 antibody [3A6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NT5DC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NT5DC1.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NT5DC1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83989)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83989)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83989)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83989)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-NT5DC1 antibody(GTX83989),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC211087 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NT5DC1 antibody(GTX83989), and then analyzed by flow cytometry.
