MA5-18287

antibody from Invitrogen Antibodies

Targeting: ARCN1 COPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-18287

Provider

Invitrogen Antibodies

Product name

COPD Monoclonal Antibody (P1318)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.

Antibody clone number

P1318

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COPD Monoclonal Antibody (P1318) detects COPD protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract . D. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. COPD Monoclonal Antibody (P1318) (Product # MA5-18287) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COPD Monoclonal Antibody (P1318) detects COPD protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw 264.7 whole cell lysate/extract. G. 30 µg C2Cl2 whole cell lysate/extract.7.5 % SDS-PAGE. COPD Monoclonal Antibody (P1318) (Product # MA5-18287) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COPD Monoclonal Antibody (P1318) detects COPD protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.7.5 % SDS-PAGE. COPD Monoclonal Antibody (P1318) (Product # MA5-18287) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COPD was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: COPD Monoclonal Antibody (P1318) (Product # MA5-18287) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of COPD was performed in paraffin-embedded mouse esophagus tissue using COPD Monoclonal Antibody (P1318) (Product # MA5-18287) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of COPD was performed in paraffin-embedded rat urinary bladder tissue using COPD Monoclonal Antibody (P1318) (Product # MA5-18287) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of COPD was performed in frozen-sectioned mouse mouse cerebellum tissue using COPD Monoclonal Antibody (P1318) (Product # MA5-18287) at a dilution of 1:250 (Green). Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.