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- Antibody Data
- Antigen structure
- References [5]
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- Western blot [1]
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- Product number
- ABIN1882266 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody
- Antibody type
- Monoclonal
- Antigen
- Purified His-tagged MGMT protein was used to produced this monoclonal antibody.
- Description
- This antibody is purified through a protein G column, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 888CT22-2-4
- Vial size
- 400 μL
- Concentration
- 0.27 mg/mL
Submitted references The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity.
Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase.
cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.
Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA.
Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.
Edara S, Goodtzova K, Pegg AE
Carcinogenesis 1995 Jul;16(7):1637-42
Carcinogenesis 1995 Jul;16(7):1637-42
Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase.
Koike G, Maki H, Takeya H, Hayakawa H, Sekiguchi M
The Journal of biological chemistry 1990 Sep 5;265(25):14754-62
The Journal of biological chemistry 1990 Sep 5;265(25):14754-62
cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.
Rydberg B, Spurr N, Karran P
The Journal of biological chemistry 1990 Jun 5;265(16):9563-9
The Journal of biological chemistry 1990 Jun 5;265(16):9563-9
Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA.
Hayakawa H, Koike G, Sekiguchi M
Journal of molecular biology 1990 Jun 20;213(4):739-47
Journal of molecular biology 1990 Jun 20;213(4):739-47
Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.
Tano K, Shiota S, Collier J, Foote RS, Mitra S
Proceedings of the National Academy of Sciences of the United States of America 1990 Jan;87(2):686-90
Proceedings of the National Academy of Sciences of the United States of America 1990 Jan;87(2):686-90
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- Experimental details
- MGMT Antibody (ABIN1882266) western blot analysis in MCF-7 cell line lysates (35 μg/lane). This demonstrates the MGMT antibody detected the MGMT protein (arrow).
