Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA805734 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA805734, RRID:AB_2627841
- Product name
- ROS1 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2)
- Antibody type
- Monoclonal
- Description
- ROS1 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ROS1
- Isotype
- IgG
- Antibody clone number
- OTI5D2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody.(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using TA805734 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min)
- Validation comment
- IHC
