Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LF-MA0033 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FDXR Monoclonal Antibody (6C2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is HeLa, Jurkat or 293T.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6C2
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ferredoxin reductase was performed by loading 20 µg of whole cell lysates from asynchronous (lane 1) and quiescent (lane 2) A549 cells, and 15 µg of plasma membrane fractions from asynchronous (lane 3) and quiescent (lane 4) A549 cells per well onto a 10% SDS-PAGE gel. Cells were made quiescent by culturing in the absence of serum for 48 hours. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in TBST for 1 hour. The membrane was probed with a Ferredoxin Reductase monoclonal antibody (Product # LF-MA0033) at a dilution of 1:500 in 2% BSA in TBST for 1 hour at room temperature, washed in TBST, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:2500 for 1 hour. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
