PA5-47837

antibody from Invitrogen Antibodies

Targeting: EPHA1 EPH, EPHT, EPHT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47837

Provider

Invitrogen Antibodies

Product name

EphA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA8, rmEphA4, recombinant rat (rr) EphA5, rmEphA2, rmEphA6, and rmEphA7 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human EphA1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for EphA1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EphA1 in T47D human breast cancer cell line. Samples were incubated in EphA1 polyclonal antibody (Product # PA5-47837) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for EphA1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of EphA1 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in EphA1 polyclonal antibody (Product # PA5-47837) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of EphA1 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in EphA1 polyclonal antibody (Product # PA5-47837) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.