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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- ELISA [4]
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- Product number
- R20181 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgM Antibody
- Antibody type
- Recombinant
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM121
- Vial size
- 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgM antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA of human plasma using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA of human IgM using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of human immunoglobulins shows the recombinant Human IgM antibody reacts only to IgM. No cross reactivity with IgG, IgE, IgD, or IgA.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgM. A serial dilution of the recombinant Human IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
