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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-48109 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MSK2 (Ser196) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL recombinant human HGF for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-human/mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48109), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MSK2 (Ser196) in HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL recombinant Human HGF for 15 minutes. Samples were incubated in Phospho-MSK2 (Ser196) polyclonal antibody (Product # PA5-48109) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Rabbit IgG secondary antibody. This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-MSK2 (Ser196) in immersion fixed paraffin-embedded sections of human pancreas. Samples were incubated in Phospho-MSK2 (Ser196) polyclonal antibody (Product # PA5-48109) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
