MAB7504

antibody from Novus Biologicals

Targeting: PRDX3 AOP-1, AOP1, MER5, SP-22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB7504

Provider

Novus Biologicals

Product name

Mouse Monoclonal Peroxiredoxin 3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Peroxiredoxin 3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Peroxiredoxin 1, 2, 4, or recombinant mouse Peroxiredoxin 3 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Peroxiredoxin 3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Peroxiredoxin 3 Monoclonal Antibody (Catalog # MAB7504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Peroxiredoxin 3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.