NB600-506AF488

antibody from Novus Biologicals

Targeting: TUBA1A B-ALPHA-1, FLJ25113, TUBA3

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-506AF488

Provider

Novus Biologicals

Product name

Rat Monoclonal alpha Tubulin Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Drosophila, Simian, Yeast

Host

Rat

Conjugate

Green dye

Antigen sequence

A linear sequence requiring an arom
atic residue at the C terminus, wit
h the two adjacent amino acids bein
g negatively charged (represented b
y Gly-Gly-Tyr in Tyr-Tubulin).


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Tubulin Antibody (YL1/2) [Alexa Fluor® 488] [NB600-506AF488] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.