- 53-4502-82 - Invitrogen Antibodies TUBA1C antibody

Submitted references Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.

Aydemirli MD, van Eendenburg JDH, van Wezel T, Oosting J, Corver WE, Kapiteijn E, Morreau H
Endocrine-related cancer 2021 May 11;28(6):377-389

Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.

Chia BS, Li B, Cui A, Eisenhaure T, Raychowdhury R, Lieb D, Hacohen N
Journal of virology 2020 Oct 27;94(22)

Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.

Addie RD, Kostidis S, Corver WE, Oosting J, Aminzadeh-Gohari S, Feichtinger RG, Kofler B, Aydemirli MD, Giera M, Morreau H
Scientific reports 2020 Jun 12;10(1):9578

CenH3-Independent Kinetochore Assembly in Lepidoptera Requires CCAN, Including CENP-T.

Cortes-Silva N, Ulmer J, Kiuchi T, Hsieh E, Cornilleau G, Ladid I, Dingli F, Loew D, Katsuma S, Drinnenberg IA
Current biology : CB 2020 Feb 24;30(4):561-572.e10

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Winkler SC, Shimobayashi E, Kapfhammer JP
Molecular neurobiology 2020 Dec;57(12):5150-5166

GATA factor-regulated solute carrier ensemble reveals a nucleoside transporter-dependent differentiation mechanism.

Zwifelhofer NM, Cai X, Liao R, Mao B, Conn DJ, Mehta C, Keles S, Xia Y, Bresnick EH
PLoS genetics 2020 Dec;16(12):e1009286

Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubules.

Chen K, Yang R, Li Y, Zhou JC, Zhang M
The Journal of cell biology 2020 Aug 3;219(8)

RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.

Gulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen J
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):E8224-E8233

Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?

Likhitpanichkul M, Torre OM, Gruen J, Walter BA, Hecht AC, Iatridis JC
Journal of biomechanics 2016 May 3;49(7):1214-1220

IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival.

Lunardi S, Jamieson NB, Lim SY, Griffiths KL, Carvalho-Gaspar M, Al-Assar O, Yameen S, Carter RC, McKay CJ, Spoletini G, D'Ugo S, Silva MA, Sansom OJ, Janssen KP, Muschel RJ, Brunner TB
Oncotarget 2014 Nov 30;5(22):11064-80

Chemotaxis of cell populations through confined spaces at single-cell resolution.

Tong Z, Balzer EM, Dallas MR, Hung WC, Stebe KJ, Konstantopoulos K
PloS one 2012;7(1):e29211

Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.

Fros JJ, Domeradzka NE, Baggen J, Geertsema C, Flipse J, Vlak JM, Pijlman GP
Journal of virology 2012 Oct;86(19):10873-9

Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization.

Mittelmeier TM, Boyd JS, Lamb MR, Dieckmann CL
The Journal of cell biology 2011 May 16;193(4):741-53

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of MDCK cells using 5 µg/mL of Anti-alpha Tubulin Alexa Fluor® 488.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor 488, eBioscience™ (Product # 53-4502-80) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e shows the isotype antibody control. The images were captured at 60X magnification.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Functional effects of IP-10 on PCCs (A) Expression of CXCR3 in PCCs, PSCs and HEK293-EBNA/CXCR3-B analyzed by Western Blot. alpha-tubulin was used as loading control. (B) Microphotographs of PCCs migrated through the Boyden chamber in response to 0.5% FBS or IP-10 (24 hr). (C-D) Viability of PCCs after IP-10 addition determined using the MTT assay. Low/high refers to cell seeding density. Data are representative of two/three independent experiments. Data in the histograms are presented as mean +- SD.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Intracellular levels of glutathione and ROS in the TCCLs, XTC.UC1, FTC-236, SW579 and BHP2-7; ( a ) ratio of reduced to oxidized glutathione, GSH/GSSG; ( b ) intracellular concentrations of GSH, GSSG and the glutathione precursors, glutamate and glycine; ( c ). uptake of glutathione precursors cysteine and serine from the culture medium (expressed as relative change, i.e. mumol/h/10 6 cells of each metabolite in each cell line relative to the mean); ( d ). intracellular levels of GSH, GSSG and serine under normal culture condition (glucose) and under glucose to galactose exchange for 6 and 24 h; ( e ). Flow cytometric analysis of ROS: left panel, hydroxyl radicals detected by CellROX, right panel superoxide radicals detected by MitoSOX. Background fluorescence (left half each panel) and after incubation with CellROX or MitoSOX, respectively (right half). Fluorescence was analysed after cells were either untreated, NAC-treated, AMA-treated or AMA + NAC-treated. f. SOD2 immunoblotting (green signal). alpha-Tubulin was used as internal reference (red signal). A short treatment with 100 uM AMA did not affect protein expression. Left lane, molecular weight markers. Top and bottom of the image were cropped. For full-length gel, see end Supplementary Information.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIG 3 RTF2 localizes to the nucleus. (A) Immunofluorescence of WT A549 cells and two distinct clones of RTF2-KO cells. (B) Biochemical fractionation of WT A549 cells into cytosolic and nuclear fractions. Cells were first suspended in HEPES-sucrose-Ficoll (HSF) buffer containing digitonin to extract the cytosolic proteins and washed once with HSF buffer, before lysing the nuclear pellet in RIPA buffer to release the nuclear proteins. TATA-binding protein (TBP), a nuclear protein, and tubulin, a cytosolic protein, were included as controls for the fractionation protocol. (C) Live-cell imaging of cells expressing either mCherry or the RTF2-mCherry fusion protein. WT A549 (top) and RTF2-KO cells (bottom) were transduced with lentiviruses that encode either FLAG-mCherry or RTF2-FLAG-mCherry and cultured for a week, before they were visualized under a wide-field epifluorescence microscope.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 10.1371/journal.pgen.1009286.g009 Fig 9 GATA factor regulatory circuit links Slc29a1 with adenosine mechanisms. (A) qRT-PCR analysis of Slc29a1 (left) and Gata2 (right) mRNA from Gata2 -77 +/+ and -77 -/- HSPCs isolated from E14.5 fetal liver (n = 10). (B) Representative Western blot analysis to detect Slc29a1 protein in Gata2 -77 +/+ and -77 -/- murine HSPCs isolated from E14.5 fetal liver (left). Quantification of relative band intensities from the immunoblots (n = 10; right). Tubulin served as a loading control. (C) qRT-PCR analysis of Slc29a1 (top) or Adk (bottom) mRNA isolated from untreated vs beta-estradiol-treated (12, 24, or 48 hours) G1E-ER-GATA1 cells. (D) Representative Western blot analysis to detect Slc29a1 and Adk protein levels in untreated vs beta-estradiol-treated (12, 24, or 48 hours) G1E-ER-GATA1 cells (left). Quantification of relative band intensities from the immunoblots (right). Tubulin served as a loading control. Statistical significance was determined by comparing values at 12, 24, or 48-hour times to those from untreated cells (0 hour). Data are from 4 separate experiments (n = 9). (E) ChIP-seq profiles of GATA1 occupancy at human (GEO GSE32491; PBDE) ADK and murine (GEO GSE30142; Ter119+ erythroblast) Adk loci [, ]. The underlined sequences denote WGATAR motifs. (F) AMP and ATP concentrations quantified by a luciferase-based assay in primary erythroblasts infected with shRNA targeting luciferase or Slc29a1 (left two panels). AMP/ATP ratio calculat
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Quantitative, multiplexed near-infrared fluorescent Western blotting. In the Western blot, the expression of proteins and phosphorylated proteins ((p)ALK as the fusion protein of EML4 or NPM1 with conformable molecular sizes, (p)STAT, (p)AKT, (p)ERK and household protein control alpha-Tubulin) for the cancer cell lines JVE404, NCI-H2228, BHP 2-7 and Karpas-299 are shown in the treated conditions of DMSO control, crizotinib (30, 100, 300 nM) and lorlatinib (30, 100, 300 nM), respectively. alphaTub, alpha-Tubulin; CR, crizotinib; LO, lorlatinib.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Interaction of CRMP2 and PKCgamma. a Immunoblot showing the co-immunoprecipitation of CRMP2 and PKCgamma from cerebellar lysates. Proteins were detected with the indicated antibodies. Normal rabbit IgG was used as a negative control. b HeLa cells were probed with alpha-PKCgamma and alpha-CRMP2 antibody and the interaction detected via Duolink proximity ligation assay (top row); alpha-CRMP2 and alpha-alpha-tubulin antibodies were used as a positive control (middle row) and primary antibodies were omitted as a negative control (bottom row). Blue, nuclei stained with DAPI; red, PLA signal. Scale bar = 20 mum
Conjugate: Green dye

53-4502-82

Antibody data