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- References [16]
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- Flow cytometry [2]
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- Product number
- NB100-1639 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1639, RRID:AB_10002125
- Product name
- Rat Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This alpha Tubulin Antibody (YOL1/34) detects an epitope on alpha tubulin between the amino acids 414-422. It has a higher affinity for fixed microtubules (formaldehyde or glutaraldehyde) than native microtubules when performing IHC.
- Reactivity
- Human, Mouse, Rat, Drosophila, Yeast
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Dendritic Cells Actively Limit Interleukin-10 Production Under Inflammatory Conditions via DC-SCRIPT and Dual-Specificity Phosphatase 4.
Mgm1 is required for maintenance of mitochondrial function and virulence in Candida albicans.
Sequence modification of the master regulator Pdr1 interferes with its transcriptional autoregulation and confers altered azole resistance in Candida glabrata.
Perturbations of NAD+ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle.
Mitochondrial complex I bridges a connection between regulation of carbon flexibility and gastrointestinal commensalism in the human fungal pathogen Candida albicans.
Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis.
Passage through the mammalian gut triggers a phenotypic switch that promotes Candida albicans commensalism.
A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony.
Ecm7, a regulator of HACS, functions in calcium homeostasis maintenance, oxidative stress response and hyphal development in Candida albicans.
Post-transcriptional regulation of the Sef1 transcription factor controls the virulence of Candida albicans in its mammalian host.
Nonredundant requirement for multiple histone modifications for the early anaphase release of the mitotic exit regulator Cdc14 from nucleolar chromatin.
Chemical-genetic profiling of imidazo[1,2-a]pyridines and -pyrimidines reveals target pathways conserved between yeast and human cells.
Phenyl hydroquinone, an Ames test-negative carcinogen, induces Hog1-dependent stress response signaling.
Mutation at the polymerase active site of mouse DNA polymerase delta increases genomic instability and accelerates tumorigenesis.
Requirement of dynactin p150(Glued) subunit for the functional integrity of the keratinocyte microparasol.
Ames test-negative carcinogen, ortho-phenyl phenol, binds tubulin and causes aneuploidy in budding yeast.
Søndergaard JN, van Heeringen SJ, Looman MWG, Tang C, Triantis V, Louche P, Janssen-Megens EM, Sieuwerts AM, Martens JWM, Logie C, Stunnenberg HG, Ansems M, Adema GJ
Frontiers in immunology 2018;9:1420
Frontiers in immunology 2018;9:1420
Mgm1 is required for maintenance of mitochondrial function and virulence in Candida albicans.
Liang C, Zhang B, Cui L, Li J, Yu Q, Li M
Fungal genetics and biology : FG & B 2018 Nov;120:42-52
Fungal genetics and biology : FG & B 2018 Nov;120:42-52
Sequence modification of the master regulator Pdr1 interferes with its transcriptional autoregulation and confers altered azole resistance in Candida glabrata.
Tian Y, Gao N, Ni Q, Mao Y, Dong D, Huang X, Jiang C, Li Z, Zhang L, Wang X, Peng Y, Chen C
FEMS yeast research 2018 Jun 1;18(4)
FEMS yeast research 2018 Jun 1;18(4)
Perturbations of NAD+ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle.
Agerholm M, Dall M, Jensen BAH, Prats C, Madsen S, Basse AL, Graae AS, Risis S, Goldenbaum J, Quistorff B, Larsen S, Vienberg SG, Treebak JT
American journal of physiology. Endocrinology and metabolism 2018 Apr 1;314(4):E377-E395
American journal of physiology. Endocrinology and metabolism 2018 Apr 1;314(4):E377-E395
Mitochondrial complex I bridges a connection between regulation of carbon flexibility and gastrointestinal commensalism in the human fungal pathogen Candida albicans.
Huang X, Chen X, He Y, Yu X, Li S, Gao N, Niu L, Mao Y, Wang Y, Wu X, Wu W, Wu J, Zhou D, Zhan X, Chen C
PLoS pathogens 2017 Jun;13(6):e1006414
PLoS pathogens 2017 Jun;13(6):e1006414
Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis.
Newnham L, Jordan PW, Carballo JA, Newcombe S, Hoffmann E
PloS one 2013;8(12):e83982
PloS one 2013;8(12):e83982
Passage through the mammalian gut triggers a phenotypic switch that promotes Candida albicans commensalism.
Pande K, Chen C, Noble SM
Nature genetics 2013 Sep;45(9):1088-91
Nature genetics 2013 Sep;45(9):1088-91
A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony.
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL
PLoS biology 2013 Sep;11(9):e1001648
PLoS biology 2013 Sep;11(9):e1001648
Ecm7, a regulator of HACS, functions in calcium homeostasis maintenance, oxidative stress response and hyphal development in Candida albicans.
Ding X, Yu Q, Xu N, Wang Y, Cheng X, Qian K, Zhao Q, Zhang B, Xing L, Li M
Fungal genetics and biology : FG & B 2013 Aug;57:23-32
Fungal genetics and biology : FG & B 2013 Aug;57:23-32
Post-transcriptional regulation of the Sef1 transcription factor controls the virulence of Candida albicans in its mammalian host.
Chen C, Noble SM
PLoS pathogens 2012;8(11):e1002956
PLoS pathogens 2012;8(11):e1002956
Nonredundant requirement for multiple histone modifications for the early anaphase release of the mitotic exit regulator Cdc14 from nucleolar chromatin.
Hwang WW, Madhani HD
PLoS genetics 2009 Aug;5(8):e1000588
PLoS genetics 2009 Aug;5(8):e1000588
Chemical-genetic profiling of imidazo[1,2-a]pyridines and -pyrimidines reveals target pathways conserved between yeast and human cells.
Yu L, Lopez A, Anaflous A, El Bali B, Hamal A, Ericson E, Heisler LE, McQuibban A, Giaever G, Nislow C, Boone C, Brown GW, Bellaoui M
PLoS genetics 2008 Nov;4(11):e1000284
PLoS genetics 2008 Nov;4(11):e1000284
Phenyl hydroquinone, an Ames test-negative carcinogen, induces Hog1-dependent stress response signaling.
Yamamoto A, Nunoshiba T, Umezu K, Enomoto T, Yamamoto K
The FEBS journal 2008 Nov;275(22):5733-44
The FEBS journal 2008 Nov;275(22):5733-44
Mutation at the polymerase active site of mouse DNA polymerase delta increases genomic instability and accelerates tumorigenesis.
Venkatesan RN, Treuting PM, Fuller ED, Goldsby RE, Norwood TH, Gooley TA, Ladiges WC, Preston BD, Loeb LA
Molecular and cellular biology 2007 Nov;27(21):7669-82
Molecular and cellular biology 2007 Nov;27(21):7669-82
Requirement of dynactin p150(Glued) subunit for the functional integrity of the keratinocyte microparasol.
Byers HR, Dykstra SG, Boissel SJ
The Journal of investigative dermatology 2007 Jul;127(7):1736-44
The Journal of investigative dermatology 2007 Jul;127(7):1736-44
Ames test-negative carcinogen, ortho-phenyl phenol, binds tubulin and causes aneuploidy in budding yeast.
Nunoshiba T, Watanabe E, Takahashi T, Daigaku Y, Ishikawa S, Mochizuki M, Ui A, Enomoto T, Yamamoto K
Mutation research 2007 Apr 1;617(1-2):90-7
Mutation research 2007 Apr 1;617(1-2):90-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tubulin Antibody (YOL1/34) [NB100-1639] - WB analysis of Tubulin and CD107b proteins on lysate of J774 cells (mouse BALB/c monocyte macrophages cell line) using different dilutions of rat anti tubulin alpha (NB100-1639) and rat anti mouse CD107b (NB100-65275) antibodies respectively with final signal detection employing goat anti rat IgG: Dylight 800.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - WB on Jurkat and THP1 cells. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - Total protein from human HeLa and A431, mouse 3T3 and rat PC12 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-alpha Tubulin in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Alpha tubulin molecular weight: 50 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): alpha Tubulin Antibody (YOL1/34) [NB100-1639] - An intracellular stain was performed on HeLa cells with Tubulin [YOL1/34] Antibody NB100-1639 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YOL1/34) [NB100-1639] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody [YOL1/34] NB100-1639AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 format of this antibody.
