PA5-75479

antibody from Invitrogen Antibodies

Targeting: A1BG

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-75479

Provider

Invitrogen Antibodies

Product name

A1BG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus.

Bernea EG, Suica VI, Uyy E, Cerveanu-Hogas A, Boteanu RM, Ivan L, Ceausu I, Mihai DA, Ionescu-Tîrgoviște C, Antohe F
Molecules (Basel, Switzerland) 2022 Aug 26;27(17)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of A1BG in Lane 1: HEK293T cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: H9C2 cell lysate. Samples were incubated with A1BG polyclonal antibody (Product # PA5-75479) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Validation by Western blotting of some protein markers identified by mass spectrometry: ( a ). Alpha-1-B Glycoprotein (A1BG) and ( b ). Haptoglobin-related protein (HPR). Representative immunoblots and densitometric analysis for each protein are shown. Ponceau S staining was used for normalization of the proteins' levels. Western blot analysis of serum exosome lysates demonstrated protein level alterations in A1BG and HPR in the GDM vs. CG comparison. Statistical analysis was performed using unpaired Student's t test, and the results were expressed as means +- standard deviation (SD); * p < 0.05. CG: control group ( n = 6) and GDM: gestational diabetes group ( n = 6).