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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 50-9714-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin E Monoclonal Antibody (HE12), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody HE12 recognizes human Cyclin E. Cyclins regulate the cell cycle by binding and activating cyclin-dependent kinases (CDK). Cyclin E regulates the transition from G1 to S phase by binding and activating CDK2. The Cyclin E/CDK2 complex is responsible for the phosphorylation of downstream proteins that facilitate the G1 to S phase transition. Overexpression of Cyclin E is found in several types of cancer including breast, colon, bladder, lung and skin. In breast cancer, Cyclin E overexpression is associated with increased stage and grade as well as poor prognosis.
- Antibody clone number
- HE12
- Concentration
- 0.2 mg/mL
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue using 10 µg/mL Mouse IgG1 K Isotype Control eFluor® 660 (left) or 10 µg/mL Anti-Human Cyclin E eFluor® 660 (right). Nuclei are stained with DAPI. Colocalization appears pink.
