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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-54360 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHGDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LEEIWPLCDF ITVHTPLLPS TTGLLNDNTF AQCKKGVRVV NCARGGIVDE GALLRALQSG QCAGAALDVF TEEPPRDRAL VDHENVISCP HLGASTKEAQ SRCGEEIAVQ FVDM
- Concentration
- 0.2 mg/mL
Submitted references SHMT2-mediated mitochondrial serine metabolism drives 5-FU resistance by fueling nucleotide biosynthesis.
Pranzini E, Pardella E, Muccillo L, Leo A, Nesi I, Santi A, Parri M, Zhang T, Uribe AH, Lottini T, Sabatino L, Caselli A, Arcangeli A, Raugei G, Colantuoni V, Cirri P, Chiarugi P, Maddocks ODK, Paoli P, Taddei ML
Cell reports 2022 Aug 16;40(7):111233
Cell reports 2022 Aug 16;40(7):111233
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHGDH in mouse cell line NIH-3T3 and rat cell line NBT-II using a PHGDH Polyclonal Antibody (Product # PA5-54360).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHGDH in human cell lines SK-MEL-30 and A-549 using a PHGDH Polyclonal Antibody (Product # PA5-54360). Corresponding PHGDH RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHGDH using PHGDH Polyclonal Antibody (Product # PA5-54360) (A) shows similar pattern to an independent PHGDH Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PHGDH in human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. Samples were probed using a PHGDH Polyclonal Antibody (Product # PA5-54360).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHGDH in human skin using a PHGDH Polyclonal Antibody (Product # PA5-54360) shows moderate cytoplasmic positivity in epidermal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHGDH in human breast cancer using a PHGDH Polyclonal Antibody (Product # PA5-54360) shows moderate to strong cytoplasmic positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHGDH in human skeletal muscle using a PHGDH Polyclonal Antibody (Product # PA5-54360) shows no positivity as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHGDH in human cerebral cortex using a PHGDH Polyclonal Antibody (Product # PA5-54360) shows moderate to strong cytoplasmic positivity in neuronal and glial cells.
