Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-32201 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32201, RRID:AB_2165386
- Product name
- Rabbit Polyclonal PHGDH Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (NBP1-32201). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.