Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF3994 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal p27/Kip1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CACYBP Enhances Cytoplasmic Retention of P27(Kip1) to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.
Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats.
Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
Antitumoral activity of lenalidomide in in vitro and in vivo models of mantle cell lymphoma involves the destabilization of cyclin D1/p27KIP1 complexes.
TRIP6 regulates p27 KIP1 to promote tumorigenesis.
Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon.
Cyclin D1 regulates p27(Kip1) stability in B cells.
Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
Homeodomain-interacting protein kinase-2 stabilizes p27(kip1) by its phosphorylation at serine 10 and contributes to cell motility.
Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
Lian YF, Huang YL, Zhang YJ, Chen DM, Wang JL, Wei H, Bi YH, Jiang ZW, Li P, Chen MS, Huang YH
Theranostics 2019;9(26):8392-8408
Theranostics 2019;9(26):8392-8408
Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats.
Theriau CF, Connor MK
Physiological reports 2017 Jul;5(13)
Physiological reports 2017 Jul;5(13)
Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
Zhao D, Besser AH, Wander SA, Sun J, Zhou W, Wang B, Ince T, Durante MA, Guo W, Mills G, Theodorescu D, Slingerland J
Oncogene 2015 Oct;34(43):5447-59
Oncogene 2015 Oct;34(43):5447-59
Antitumoral activity of lenalidomide in in vitro and in vivo models of mantle cell lymphoma involves the destabilization of cyclin D1/p27KIP1 complexes.
Moros A, Bustany S, Cahu J, Saborit-Villarroya I, Martínez A, Colomer D, Sola B, Roué G
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 15;20(2):393-403
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 15;20(2):393-403
TRIP6 regulates p27 KIP1 to promote tumorigenesis.
Lin VT, Lin VY, Lai YJ, Chen CS, Liu K, Lin WC, Lin FT
Molecular and cellular biology 2013 Apr;33(7):1394-409
Molecular and cellular biology 2013 Apr;33(7):1394-409
Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
Uranbileg B, Yamamoto H, Park JH, Mohanty AR, Arakawa-Takeuchi S, Jinno S, Okayama H
The Journal of biological chemistry 2012 Feb 24;287(9):6275-83
The Journal of biological chemistry 2012 Feb 24;287(9):6275-83
A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
Dumitru CA, Fechner MK, Hoffmann TK, Lang S, Brandau S
Journal of leukocyte biology 2012 Apr;91(4):591-8
Journal of leukocyte biology 2012 Apr;91(4):591-8
Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
Wen S, So Y, Singh K, Slingerland JM, Resnick MB, Zhang S, Ruiz V, Moss SF
Oncogene 2012 Apr 5;31(14):1771-80
Oncogene 2012 Apr 5;31(14):1771-80
The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon.
Donohoe DR, Garge N, Zhang X, Sun W, O'Connell TM, Bunger MK, Bultman SJ
Cell metabolism 2011 May 4;13(5):517-26
Cell metabolism 2011 May 4;13(5):517-26
Cyclin D1 regulates p27(Kip1) stability in B cells.
Bustany S, Tchakarska G, Sola B
Cellular signalling 2011 Jan;23(1):171-9
Cellular signalling 2011 Jan;23(1):171-9
Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
Santi SA, Lee H
PloS one 2011 Jan 31;6(1):e14614
PloS one 2011 Jan 31;6(1):e14614
Homeodomain-interacting protein kinase-2 stabilizes p27(kip1) by its phosphorylation at serine 10 and contributes to cell motility.
Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A
The Journal of biological chemistry 2011 Aug 19;286(33):29005-13
The Journal of biological chemistry 2011 Aug 19;286(33):29005-13
Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):81-90
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):81-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-p27/Kip1 (T198) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.