PA5-47160

antibody from Invitrogen Antibodies

Targeting: EDAR DL, ED1R, ED3, ED5, EDA1R, EDA3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47160

Provider

Invitrogen Antibodies

Product name

EDAR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In Western blots, less than 1% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR6, rhTAJ, rhTNF RI, rhTNF RII, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGFR, rhOPG and rhRANK is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-EDAR Polyclonal Antibody (Product # PA5-47160) and a 60 kDa band corresponding to EDAR was observed in HeLa and HEK-293. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and HEK-293 (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1µg/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).