NBP1-49908

antibody from Novus Biologicals

Targeting: F11R CD321, JAM-1, JAM-A, JAM1, JAMA, JCAM, PAM-1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-49908

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-49908, RRID:AB_10012098

Product name

Rabbit Polyclonal JAM-A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: JAM-A Antibody [NBP1-49908] - Samples: Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody NBP1-49908 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. JAM-A was not successfully immunoprecipitated by rabbit anti-JAM-A antibody NBP1-49907, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: JAM-A Antibody [NBP1-49908] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A used at a dilution of 1:200 (1ug/ml). Detection: DAB