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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX115554 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115554, RRID:AB_10624806
- Product name
- RbAp48 antibody [N1C2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Nuclear proteomics with XRCC3 knockdown to reveal the development of doxorubicin-resistant uterine cancer.
Chang JF, Lin ST, Hung E, Lu YL, Soon May EW, Lo YW, Chou HC, Chan HL
Toxicological sciences : an official journal of the Society of Toxicology 2014 Jun;139(2):396-406
Toxicological sciences : an official journal of the Society of Toxicology 2014 Jun;139(2):396-406
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE GTX115554 diluted at 1:3000
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- GeneTex (provider)
- Main image
- Experimental details
- SH-SY5Y whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [N1C2] (GTX115554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- RbAp48 antibody [N1C2] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [N1C2] (GTX115554) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
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- GeneTex (provider)
- Main image
- Experimental details
- RbAp48 antibody [N1C2] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [N1C2] (GTX115554) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RbAp48(GTX115554) antibody at 1:500 dilution.
