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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-30422 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RbAp48 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, SH-SY5Y, SH-SY5Y nuclear extract.
- Concentration
- 0.28 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RbAp48 was performed by separating 30 µg of SH-SY5Y whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RbAp48 Polyclonal Antibody (Product # PA5-30422) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RbAp48 Polyclonal Antibody (Product # PA5-30422). Sample (30 µg of whole cell lysate). Lane A: JurKat. 10% SDS PAGE. RbAp48 Polyclonal Antibody (Product # PA5-30422) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RbAp48 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp48 Polyclonal Antibody (Product # PA5-30422) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RbAp48 was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp48 Polyclonal Antibody (Product # PA5-30422) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RbAp48 (Product # PA5-30422) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
