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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-41888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XRN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: LPQEISQVNQ HHKSGFNDNS VKYQQRKHDP HRKFKEECKS PKAECWSQKM
- Concentration
- 0.5 mg/mL
Submitted references Elevated Wall Tension Leads to Reduced miR-133a in the Thoracic Aorta by Exosome Release.
Akerman AW, Blanding WM, Stroud RE, Nadeau EK, Mukherjee R, Ruddy JM, Zile MR, Ikonomidis JS, Jones JA
Journal of the American Heart Association 2019 Jan 8;8(1):e010332
Journal of the American Heart Association 2019 Jan 8;8(1):e010332
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF-7 cell lysate using an anti-XRN1 polyclonal antibody (Product # PA5-41888).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Mechanical tension and micro RNA- specific ribonuclease mRNA expression and protein abundance. Total RNA or protein was isolated from each independent cell line and examined by real-time polymerase chain reaction or Western blotting, respectively. A , XRN 1 mRNA expression levels in aortic fibroblasts exposed to 12% biaxial cyclic stretch (Tension, n=9) or held static (Control, n=9) for 3 hours. B , XRN 1 protein abundance and representative immunoblot in aortic fibroblasts exposed to 12% biaxial cyclic stretch (Tension, n=5) or held static (Control, n=5) for 3 hours. C , XRN 2 mRNA expression levels in aortic fibroblasts exposed to 12% biaxial cyclic stretch (Tension, n=9) or held static (Control, n=9) for 3 hours. D , XRN 2 protein abundance and representative immunoblot in aortic fibroblasts exposed to 12% biaxial cyclic stretch (Tension, n=5) or held static (Control, n=5) for 3 hours. E , Exo SC 4 mRNA expression levels in aortic fibroblasts exposed to 12% biaxial cyclic stretch (Tension, n=9) or held static (Control, n=9) for 3 hours. F , Exo SC 4 protein abundance and representative immunoblot in aortic fibroblasts exposed to 12% biaxial cyclic stretch (Tension, n=5) or held static (Control, n=5) for 3 hours. A through F , Data are represented in dot plots with the mean and SEM shown next to each group. Comparisons were made using a 2-sample t test (unpaired, 2 tailed). No significant differences were observed vs control.
