MAB5010

antibody from Novus Biologicals

Targeting: IL33 C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5010

Provider

Novus Biologicals

Product name

Rat Monoclonal IL-33 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-33 Propeptide in direct ELISAs and Western blots. In Western blots, no cross-reactivity with mature recombinant mouse IL-33, mature recombinant human (rh) IL-33, or the pro region of rhIL-33 is observed.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.

Ohno T, Oboki K, Morita H, Kajiwara N, Arae K, Tanaka S, Ikeda M, Iikura M, Akiyama T, Inoue J, Matsumoto K, Sudo K, Azuma M, Okumura K, Kamradt T, Saito H, Nakae S
PloS one 2011 Apr 11;6(4):e18404

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse IL-33 Propeptide by Western Blot. Western blot shows lysates of mouse peripheral lymph node tissue under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Mouse IL-33 Propeptide Monoclonal Antibody (Catalog # MAB5010) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for IL-33 Propeptide was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.