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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Flow cytometry [1]
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- Product number
- IC3626P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-33 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-33 in direct ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Antigen sequence
Q8BVZ5- Isotype
- IgG
- Antibody clone number
- 396118
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms.
IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis.
Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33.
IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
Murine mast cells secrete and respond to interleukin-33.
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection.
Yamaguchi M, Samuchiwal SK, Quehenberger O, Boyce JA, Balestrieri B
Mucosal immunology 2018 May;11(3):615-626
Mucosal immunology 2018 May;11(3):615-626
IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis.
Chen S, Chen B, Wen Z, Huang Z, Ye L
Oncotarget 2017 May 16;8(20):32407-32418
Oncotarget 2017 May 16;8(20):32407-32418
Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan DA, Santillan MK, Zaheer A
PloS one 2015;10(8):e0135776
PloS one 2015;10(8):e0135776
Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33.
Verma A, Kroetz DN, Tweedle JL, Deepe GS Jr
Mucosal immunology 2015 Mar;8(2):380-9
Mucosal immunology 2015 Mar;8(2):380-9
IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
Guabiraba R, Besnard AG, Menezes GB, Secher T, Jabir MS, Amaral SS, Braun H, Lima-Junior RC, Ribeiro RA, Cunha FQ, Teixeira MM, Beyaert R, Graham GJ, Liew FY
Mucosal immunology 2014 Sep;7(5):1079-93
Mucosal immunology 2014 Sep;7(5):1079-93
Murine mast cells secrete and respond to interleukin-33.
Tung HY, Plunkett B, Huang SK, Zhou Y
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 Mar;34(3):141-7
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 Mar;34(3):141-7
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
Mizutani N, Nabe T, Yoshino S
Immunology 2013 Jun;139(2):205-18
Immunology 2013 Jun;139(2):205-18
Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection.
Wills-Karp M, Rani R, Dienger K, Lewkowich I, Fox JG, Perkins C, Lewis L, Finkelman FD, Smith DE, Bryce PJ, Kurt-Jones EA, Wang TC, Sivaprasad U, Hershey GK, Herbert DR
The Journal of experimental medicine 2012 Mar 12;209(3):607-22
The Journal of experimental medicine 2012 Mar 12;209(3):607-22
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 PE-conjugated Mono-clonal Antibody (Catalog # IC3626P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
