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- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB3626 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-33 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-33 in direct ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BVZ5- Isotype
- IgG
- Antibody clone number
- 396118
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells.
Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection.
Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration.
IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
The alarmin IL-33 promotes regulatory T-cell function in the intestine.
Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis.
α-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation.
Inducible IL-33 expression by mast cells is regulated by a calcium-dependent pathway.
IL-33 attenuates EAE by suppressing IL-17 and IFN-γ production and inducing alternatively activated macrophages.
Production and functions of IL-33 in the central nervous system.
IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
Induction of IL-33 expression and activity in central nervous system glia.
Kubo F, Ariestanti DM, Oki S, Fukuzawa T, Demizu R, Sato T, Sabirin RM, Hirose S, Nakamura N
Respiratory research 2019 Jan 17;20(1):11
Respiratory research 2019 Jan 17;20(1):11
Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection.
Qi F, Bai S, Wang D, Xu L, Hu H, Zeng S, Chai R, Liu B
Molecular immunology 2017 Jul;87:284-292
Molecular immunology 2017 Jul;87:284-292
Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
Liu T, Kanaoka Y, Barrett NA, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw TM, Katz HR, Boyce JA
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3537-45
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3537-45
IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration.
Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams HP, Lou Y
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2140-7
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2140-7
IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
Guabiraba R, Besnard AG, Menezes GB, Secher T, Jabir MS, Amaral SS, Braun H, Lima-Junior RC, Ribeiro RA, Cunha FQ, Teixeira MM, Beyaert R, Graham GJ, Liew FY
Mucosal immunology 2014 Sep;7(5):1079-93
Mucosal immunology 2014 Sep;7(5):1079-93
The alarmin IL-33 promotes regulatory T-cell function in the intestine.
Schiering C, Krausgruber T, Chomka A, Fröhlich A, Adelmann K, Wohlfert EA, Pott J, Griseri T, Bollrath J, Hegazy AN, Harrison OJ, Owens BMJ, Löhning M, Belkaid Y, Fallon PG, Powrie F
Nature 2014 Sep 25;513(7519):564-568
Nature 2014 Sep 25;513(7519):564-568
Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
Chen F, Wu W, Millman A, Craft JF, Chen E, Patel N, Boucher JL, Urban JF Jr, Kim CC, Gause WC
Nature immunology 2014 Oct;15(10):938-46
Nature immunology 2014 Oct;15(10):938-46
Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis.
Carlock CI, Wu J, Zhou C, Tatum K, Adams HP, Tan F, Lou Y
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):161-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):161-9
α-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
Huang JR, Tsai YC, Chang YJ, Wu JC, Hung JT, Lin KH, Wong CH, Yu AL
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1972-81
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1972-81
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
Mizutani N, Nabe T, Yoshino S
Immunology 2013 Jun;139(2):205-18
Immunology 2013 Jun;139(2):205-18
Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation.
Juncadella IJ, Kadl A, Sharma AK, Shim YM, Hochreiter-Hufford A, Borish L, Ravichandran KS
Nature 2013 Jan 24;493(7433):547-51
Nature 2013 Jan 24;493(7433):547-51
Inducible IL-33 expression by mast cells is regulated by a calcium-dependent pathway.
Hsu CL, Bryce PJ
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3421-9
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3421-9
IL-33 attenuates EAE by suppressing IL-17 and IFN-γ production and inducing alternatively activated macrophages.
Jiang HR, Milovanović M, Allan D, Niedbala W, Besnard AG, Fukada SY, Alves-Filho JC, Togbe D, Goodyear CS, Linington C, Xu D, Lukic ML, Liew FY
European journal of immunology 2012 Jul;42(7):1804-14
European journal of immunology 2012 Jul;42(7):1804-14
Production and functions of IL-33 in the central nervous system.
Yasuoka S, Kawanokuchi J, Parajuli B, Jin S, Doi Y, Noda M, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A
Brain research 2011 Apr 18;1385:8-17
Brain research 2011 Apr 18;1385:8-17
IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
Hsu CL, Neilsen CV, Bryce PJ
PloS one 2010 Aug 3;5(8):e11944
PloS one 2010 Aug 3;5(8):e11944
Induction of IL-33 expression and activity in central nervous system glia.
Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
Journal of leukocyte biology 2008 Sep;84(3):631-43
Journal of leukocyte biology 2008 Sep;84(3):631-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse IL-33 by Western Blot. Western blot shows lysates of mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for IL-33 at approximately 18 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-33 in bEnd.3 Mouse Cell Line. IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel, Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
