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LearnPA5-20398
antibody from Invitrogen Antibodies
Targeting: IL33
C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-20398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-33 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The PA5-20398 immunogen is located within the 150-200 amino acids of IL-33.
- Concentration
- 1.54 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human lymph node tissue lysate using a IL-33 polyclonal antibody (Product # PA5-20398) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation of IL-33 in A20 Cells. Loading: 15 µg of lysates per lane. Antibodies: IL-33 Polyclonal Antibody (Product # PA5-20398) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane A: 1 µg/mL Lane B: 2 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation of IL-33 in Human Lung. Loading: 15. µg of human lung lysate Antibodies: IL-33 Polyclonal Antibody (Product # PA5-20398) 2 µg/mL , 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation of IL-33 in Human Lymph Node. Loading: 15. µg of human lymph node lysate Antibodies: IL-33 Polyclonal Antibody (Product # PA5-20398) 2 µg/mL , 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation of IL-33 with Human Recombinant Protein. Loading: 5 ng (1) and 25 ng (2) of IL-33 partial human recombinant protein. Antibodies: IL-33 Polyclonal Antibody (Product # PA5-20398) 1 µg/mL , 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Il-33 partial human recombinant protein: 112-270aa, 17.9 kD, 36 kD band is the dimer.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human lymph node cells using a IL-33 polyclonal antibody (Product # PA5-20398) at a 20 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human lymph node tissue labeling IL-33 with IL-33 Polyclonal Antibody (Product # PA5-20398) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lymph node tissue using IL-33 Polyclonal Antibody (Product # PA5-20398) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
