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Learn710268
antibody from Invitrogen Antibodies
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- 710268 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAFF Recombinant Polyclonal Antibody (12HCLC)
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is predicted to react with equine, non-human primate and rabbit based on sequence homology.
- Antibody clone number
- 12HCLC
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAFFin whole cell extracts from Jurkat using a BAFFRecombinant Rabbit Polyclonal Antibody (Product # 710268) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~31kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP1 (Lane 1), Raji (Lane 2), Jurkat (Lane 3), PC-3 (Lane 4), MCF 7 (Lane 5), HEK 293 (Lane 6) and K562 (Lane 7). The blots were probed with Anti-BAFF Recombinant Rabbit Polyclonal Antibody (Product # 710268, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:2500 dilution). A ~ 29 kDa band corresponding to BAFF was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAFFin whole cell extracts from Jurkat using a BAFFRecombinant Rabbit Polyclonal Antibody (Product # 710268) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~31kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BAFF/CD257 showing staining in the cytoplasm and nucleus of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a BAFF/CD257 Recombinant Rabbit Polyclonal Antibody (Product # 710268) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
