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LearnIC1241P
antibody from R&D Systems
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC1241P - Provider product page
- Provider
- R&D Systems
- Product name
- Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TL1A/TNSF15 is observed and no cross-reactivity with rhAPRIL, rhTNF-alpha , rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTRAIL, rhTRANCE, or rhTWEAK is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q9Y275- Isotype
- IgG
- Antibody clone number
- 137314
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.
Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
The British journal of dermatology 2012 Aug;167(2):359-67
The British journal of dermatology 2012 Aug;167(2):359-67
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.
Nishio M, Endo T, Tsukada N, Ohata J, Kitada S, Reed JC, Zvaifler NJ, Kipps TJ
Blood 2005 Aug 1;106(3):1012-20
Blood 2005 Aug 1;106(3):1012-20
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of BAFF/BLyS/TNFSF13B in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin (filled histogram) or resting (open histogram), were stained with Mouse Anti-Human BAFF/BLyS/TNFSF13B PE-conjugated Monoclonal Antibody (Catalog # IC1241P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
