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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB626 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TRANCE/TNFSF11/RANK L Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects TRANCE/TNFSF11/RANK L in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse TRANCE is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/TNFSF11/ RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRANCE/ TNFSF11/RANK L Polyclonal Antibody (Catalog # AB626). The ND50 is typically 5-15 µg/mL.
