Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319469 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-RANK-L antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-RANK-L antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Tnfsf11
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Anti-mouse RANKL antibody in WB shows detection of recombinant mouse RANKL raised in E.coli. Recombinant truncated protein (0.1 ug, 19.9 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was incubated with Anti-Mouse RANKL antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight„? 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000.
- Validation comment
- WB