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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA1-41161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD254 (RANK Ligand) Monoclonal Antibody (12A668)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: activated T cells or RAW, antigen standard for TNFSF11 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12A668
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats.
Saglam E, Alinca SB, Celik TZ, Hacisalihoglu UP, Dogan MA
Journal of applied oral science : revista FOB 2020;28:e20190140
Journal of applied oral science : revista FOB 2020;28:e20190140
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Trance (RANKL) in Transfected cell lysate was probed with 2 µg of a CD254/RANKL monoclonal antibody (Product # MA1-41161).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD254 (RANK Ligand) in 35 µg/lane human lymph node lysate. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA1-41161 using a dilution of 0.5 µg/mL. Band detected at ~35 kDa and ~28 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD254 (RANK Ligand) in 2 µg transfected cell lysate. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA1-41161.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CD254 (RANK Ligand) in immersion fixed mouse splenocytes. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA1-41161). Incubated for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies and counterstained with DAPI (blue). Image using the standard format of this product.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD254 (RANK Ligand) in formalin-fixed, paraffin-embedded human lymph node. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA1-41161) using a dilution of 5 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD254 (RANK Ligand) in formalin-fixed, paraffin-embedded human liver. Samples were incubated in CD254 (RANK Ligand) monoclonal antibody (Product # MA1-41161) using a dilution of 1:500, 1 µg/mL followed by peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.
