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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NB100-56748 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56748, RRID:AB_839048
- Product name
- Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3.
MMP14 is a novel target of PTH signaling in osteocytes that controls resorption by regulating soluble RANKL production.
The interleukin-20 receptor axis in early rheumatoid arthritis: novel links between disease-associated autoantibodies and radiographic progression.
RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model.
Expression of bone-regulatory proteins in human valve allografts.
Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis.
Lei L, Huang Z, Feng J, Huang Z, Tao Y, Hu X, Zhang X
Stem cell research & therapy 2020 Mar 26;11(1):137
Stem cell research & therapy 2020 Mar 26;11(1):137
MMP14 is a novel target of PTH signaling in osteocytes that controls resorption by regulating soluble RANKL production.
Delgado-Calle J, Hancock B, Likine EF, Sato AY, McAndrews K, Sanudo C, Bruzzaniti A, Riancho JA, Tonra JR, Bellido T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May;32(5):2878-2890
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May;32(5):2878-2890
The interleukin-20 receptor axis in early rheumatoid arthritis: novel links between disease-associated autoantibodies and radiographic progression.
Kragstrup TW, Greisen SR, Nielsen MA, Rhodes C, Stengaard-Pedersen K, Hetland ML, Hørslev-Petersen K, Junker P, Østergaard M, Hvid M, Vorup-Jensen T, Robinson WH, Sokolove J, Deleuran B
Arthritis research & therapy 2016 Mar 11;18:61
Arthritis research & therapy 2016 Mar 11;18:61
RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model.
Walker CG, Ito Y, Dangaria S, Luan X, Diekwisch TG
European journal of oral sciences 2008 Aug;116(4):312-8
European journal of oral sciences 2008 Aug;116(4):312-8
Expression of bone-regulatory proteins in human valve allografts.
Shetty R, Pepin A, Charest A, Perron J, Doyle D, Voisine P, Dagenais F, Pibarot P, Mathieu P
Heart (British Cardiac Society) 2006 Sep;92(9):1303-8
Heart (British Cardiac Society) 2006 Sep;92(9):1303-8
Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis.
Yoneda T, Ishimaru N, Arakaki R, Kobayashi M, Izawa T, Moriyama K, Hayashi Y
Endocrinology 2004 May;145(5):2384-91
Endocrinology 2004 May;145(5):2384-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody. Image using the PE format of this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.
