Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA1-9010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYCARD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of PYCARD in U937 lysate using Product # PA1-9010.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYCARD by a PYCARD monoclonal antibody (Product # PA1-9010) at a concentration of 2 µg/mL. U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of PYCARD in U937 lysate using Product # PA1-9010.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PYCARD in Human Tonsil using a PYCARD monoclonal antibody (Product #PA1-9010) at 2.5 µg/mL. The Human Tonsil tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.