Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-19957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYCARD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is HL60 cell lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide using a ASC polyclonal antibody (Product # PA5-19957) at 1 µg /mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human HL60 Cells. Loading: 15 µg of lysates per lane. Antibodies: PYCARD Polyclonal Antibody (Product # PA5-19957) (1 µg/mL) in the absence (A) or presence (B) of blocking peptide, 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human THP-1 Cells. Loading: 15 µg of lysate per lane. Antibodies: PYCARD Polyclonal Antibody (Product # PA5-19957) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of HL60 cells using PYCARD Polyclonal Antibody (Product # PA5-19957) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HL60 cells using a ASC polyclonal antibody (Product # PA5-19957) at a 5 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with PYCARD Polyclonal Antibody (Product # PA5-19957) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with PYCARD Polyclonal Antibody (Product # PA5-19957) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human spleen tissue using PYCARD Polyclonal Antibody (Product # PA5-19957) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.