TA503885

antibody from OriGene

Targeting: PDIA4 ERP70, ERP72

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503885

Provider

OriGene

Proper citation

OriGene Cat#TA503885, RRID:AB_11124594

Product name

PDIA4 (ERp72) mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)

Antibody type

Monoclonal

Description

PDIA4 (ERp72) mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)

Host

Mouse

Conjugate

Unconjugated

Epitope

PDIA4

Isotype

IgG

Antibody clone number

OTI2B11

Vial size

100 µl

Concentration

0.45 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA4 (RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PDIA4 mouse monoclonal antibody (TA503885) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDIA4(RC204041).

Validation comment

IF