MA5-34919

antibody from Invitrogen Antibodies

Targeting: PDIA4 ERP70, ERP72

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34919

Provider

Invitrogen Antibodies

Product name

ERp72 Monoclonal Antibody (10-F2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: A431, HepG2, SiHa, SKOV-3, human lung cancer tissue, human liver tissue, rat testis tissue, human placenta tissue, mouse brain tissue.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10-F2

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ERp72 in A431 cell lysate. Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ERp72 in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ERp72 monoclonal antibody (Product # MA5-34919), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ERp72 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ERp72 monoclonal antibody (Product # MA5-34919), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ERp72 in paraffin-embedded rat testis tissue. Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ERp72 in paraffin-embedded mouse brain tissue. Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ERp72 in paraffin-embedded human placenta tissue. Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ERp72 in paraffin-embedded human lung cancer tissue. Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ERp72 in paraffin-embedded human liver tissue. Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ERp72 in SiHa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with ERp72 monoclonal antibody (Product # MA5-34919) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG.