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PA5-29910

antibody from Invitrogen Antibodies

Targeting: GSN DKFZp313L0718

Provider product page for PA5-29910

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [5]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Validation data

Product number: PA5-29910 - Provider product page
Provider: Invitrogen Antibodies
Product name: Gelsolin Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: H1299, HeLa, human plasma, mouse plasma, rat plasma.
Concentration: 0.13 mg/mL

GSN protein structure - PA5-29910 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Gelsolin in Human tissue extract (30 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Gelsolin Polyclonal antibody (Product # PA5-29910) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Gelsolin was performed by separating 30 µg of Human tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Gelsolin Polyclonal Antibody (Product # PA5-29910) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Gelsolin Polyclonal Antibody (Product # PA5-29910). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: HeLa. 7.5% SDS PAGE. Gelsolin Polyclonal Antibody (Product # PA5-29910) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Gelsolin was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Gelsolin Polyclonal Antibody (Product # PA5-29910) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Gelsolin was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Gelsolin Polyclonal Antibody (Product # PA5-29910) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.