LS-C744789

antibody from LSBio

Targeting: FGA

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C744789

Provider

LSBio

Product name

FGA / Fibrinogen Alpha Antibody (Liquid, Biotin) LS-C744789

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of Goat anti-Fibrinogen antibody. Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of Goat Anti-Fibrinogen antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot of Goat anti-Fibrinogen antibody. Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT.