ABIN499148

antibody from antibodies-online

Targeting: PRKAA2 AMPK, AMPKa2, PRKAA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN499148

Provider

antibodies-online

Product name

anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (Internal Region) antibody

Antibody type

Polyclonal

Antigen

Peptide with sequence from the internal region of the protein sequence according to NP_006243.2. Genename: PRKAA2

Description

Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Goat

Antigen sequence

CPLDALNTTKP


Epitope

Internal Region

Vial size

0.1 mg

Concentration

0.5 mg/mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Reduction of AMP-activated protein kinase alpha2 increases endoplasmic reticulum stress and atherosclerosis in vivo.

Dong Y, Zhang M, Liang B, Xie Z, Zhao Z, Asfa S, Choi HC, Zou MH
Circulation 2010 Feb 16;121(6):792-803

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

AP21300PU-N AMPK2 antibody staining of Human Liver lysate at 1μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

AP21300PU-N AMPK2 antibody staining of Mouse (A) and Rat (B) Heart lysate at 0.3 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ELISA

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

AP21300PU-N (1.5 μg/ml) as the reporter with a capture Rabbit antibody (2.5 μg/ml).