PAB8141

antibody from Abnova Corporation

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB8141

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB8141, RRID:AB_1676187

Product name

AKT3 (phospho S472) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells.

Xu Z, Ma DZ, Wang LY, Su JM, Zha XL
Biochemical and biophysical research communications 2003 Dec 12;312(2):388-96

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Defective signaling through Akt-2 and -3 but not Akt-1 in insulin-resistant human skeletal muscle: potential role in insulin resistance.

Brozinick JT Jr, Roberts BR, Dohm GL
Diabetes 2003 Apr;52(4):935-41

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Gene expression profiling in prostate cancer cells with Akt activation reveals Fra-1 as an Akt-inducible gene.

Tiwari G, Sakaue H, Pollack JR, Roth RA
Molecular cancer research : MCR 2003 Apr;1(6):475-84

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of AKT3 (phospho S472) polyclonal antibody (Cat # PAB8141) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)