LS-C744908

antibody from LSBio

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot

ELISA

Immunocytochemistry

Antibody data

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Validation data

Product number: LS-C744908 - Provider product page
Provider: LSBio
Product name: AKT3 Antibody (Internal, clone 25F6.F6.D8, FITC) LS-C744908
Antibody type: Monoclonal
Description: Protein A affinity chromatography
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Green dye
Isotype: IgG
Antibody clone number: 25F6.F6.D8
Storage: Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

AKT3 protein structure - LS-C744908 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of Mouse Monoclonal anti AKT3 unconjugated Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: 2ug/ml 1.5 hour @ room T. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45” RT.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 unconjugated antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of Mouse Monoclonal anti AKT3 unconjugated Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: 2ug/ml 1.5 hour @ room T. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45” RT.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 unconjugated antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.